Msat Upstream Scientist

3 weeks ago


Singapore SGVECTOR PTE. LTD. Full time

Job responsibilities:

  • Perform plasmid cloning, transformation, amplification, and purification.
  • Maintain mammalian cell cultures using aseptic techniques.
  • Setup and operate benchtop (2L) and largescale single use bioreactors (50L)
  • Utilize DoE methodology to optimize cell growth and viral vector production in suspension mammalian cell cultures.
  • Collaborate with team members to identify and evaluate process improvements with the goal of optimizing the current AAV and LVV production process.
  • Perform molecular biology and analytical assays such as SDS-PAGE, Western blot, flow cytometry, ELISA and qPCR.
  • Draft lab protocols, standard operating procedures, sampling plans, technical reports, and batch records for regulatory and tech transfer purposes.
  • Search literature for new methods, processes, and assess new equipment and technologies in order to expand and improve upstream AAV and LVV capabilities.

Requirements:

  • Tertiary degree (Bachelor, Master or Phd) in Chemical Engineering, Pharmaceutical Engineering, Biomedical Engineering, Cell and Molecular Biology, Biochemistry, Biotechnology or related fields with at least 24 years of relevant laboratory and/or industry experience in Cell and Gene Therapy.
  • Handson experience with mammalian cell expression technology and upstream bioprocess optimization, using suspensionbased bioreactors is required.
  • Prior experience in process development or GMP manufacturing of viral vector products.
  • Experience in aseptic techniques and working in a BSL2 environment.
  • Highly motivated team player with an ability to be productive and a willingness to learn in a fluid, fastpaced and environment are essential.
  • Excellent organizational and collaborative skills, with the ability to design and troubleshoot experiments with mínimal supervision or as part of a group.

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