Scientist Upstream Process Development

5 days ago

Singapore SGVECTOR PTE. LTD. Full time

**Job Description**:

- Perform plasmid cloning, transformation, amplification, and purification.
- Maintain mammalian cell cultures using aseptic techniques.
- Set-up and operate benchtop and large-scale single use bioreactors (50 to 100L)
- Utilize DoE methodology to optimize cell growth and viral vector titers in suspension mammalian cell cultures.
- Collaborate with team members to identify and evaluate process improvements with the goal of optimizing the current rAAV production process.
- Perform molecular biology and analytical assays to support the upstream and downstream PD efforts.
- Draft and edit lab protocols, standard operating procedures, sampling plans, technical documents, and batch records for regulatory or tech transfer purposes.
- Search literature for methods, processes, and assess new equipment and technologies in order to expand and improve upstream AAV capabilities.
- Work with senior team members to perform and understand process experiments and characterization studies.

**Requirements**:

- Master or Bachelor’s degree with ~6 years or more relevant industry experience in Cell and Molecular Biology, Biomedical engineering, Biochemistry, Biotechnology, Chemical Engineering or related fields.
- Considerable direct experience with mammalian cell expression technology and media optimization, utilizing suspension-based bioreactor systems is required.
- Prior experience in viral vector GMP manufacturing, process characterization and QA/QC release testing of viral vector products are required.
- Experience working in a BSL-2 environment and using aseptic techniques.
- Hands-on experience with bench-scale bioreactor operations and large-scale single use bioreactors (50 to 100L) are required.
- Proficient in general molecular biology techniques such as SDS-PAGE, Western blot, flow cytometry, ELISA, ddPCR, qPCR, PCR and cell-based potency assays.
- Experience in drafting study plans, technical reports, and protocols.
- A highly motivated team player with an ability to be productive and a willingness to learn in a fluid, fast-paced and environment are essential.
- Excellent organizational and collaborative skills, with the ability to design and troubleshoot experiments with mínimal supervision or as part of a group.

  • Scientist - Bioprocessing Upstream

    2 weeks ago

    Singapore WUXI ADVANCED THERAPIES SINGAPORE PTE. LTD. Full time

    **Company Overview: **WuXi Advanced Therapies**: - Accelerating Progress and Time to Market - Integrated Manufacturing and Testing Service Platform - Solutions From Gene of Interest to Global Commercial Launch The advanced therapies business unit of WuXi AppTec is a leading cell and gene therapy Contract Testing, Development and Manufacturing Organization...

  • Upstream Field Applications Scientist, Singapore

    6 days ago

    Singapore Repligen Corporation Full time

    Overview Repligen is a bioprocessing-focused life sciences company bringing over 30 years of expertise and innovation to our customers. We are inspiring advances in bioprocessing through the development and commercialization of high-value products and flexible solutions that address critical steps in the production of biologic drugs. We are seeking a...

  • Upstream Field Applications Scientist, Singapore

    1 week ago

    Singapore Repligen Full time

    Overview: Repligen is a bioprocessing-focused life sciences company bringing over 30 years of expertise and innovation to our customers. We are inspiring advances in bioprocessing through the development and commercialization of high-value products and flexible solutions that address critical steps in the production of biologic drugs. We are seeking a...

  • Upstream Field Applications Scientist, Singapore

    1 week ago

    Singapore Repligen Corporation Full time

    **Overview**: - Repligen is a bioprocessing-focused life sciences company bringing over 30 years of expertise and innovation to our customers. We are inspiring advances in bioprocessing through the development and commercialization of high-value products and flexible solutions that address critical steps in the production of biologic drugs. We are seeking...

  • Upstream Process Development, Scientist I

    2 weeks ago

    Singapore VALERE SG PTE. LTD. Full time

    **Responsibilities** - Assist in design and execution of cloning experiments, including plasmid construction and transformation. - Execute studies for pre-CMC and CMC production by evaluating different microbial strains, optimizing fermentation processes, constructing plasmids, banking cells, and conducting microbial fermentations up to 10L scale. - Gain...

  • Associate Scientist

    2 weeks ago

    Singapore NUSANTARA PRIME CONSULTING PTE LTD Full time

    **Responsibilities**: - Assist establishment of Drug Substance process development, support and troubleshoot biologics commercial upstream / downstream manufacturing processes - Plan, design, execute and interpretation of lab experiments to support plant operations, troubleshooting, process mapping, process transfer, process improvements and/or the...

  • Downstream Process Development

    2 days ago

    Singapore NUEVOCOR PTE. LTD. Full time

    **Job description** Nuevocor is a pre-clinical stage biopharmaceutical start-up dedicated to developing gene therapies to treat the underlying cause of genetic cardiomyopathies. Backed by an international syndicate of reputable venture capital firms, it will focus on advancing its lead program to clinical trials. It will also leverage its platform...

  • Upstream Bioprocessing Specialist

    7 hours ago

    Singapore beBeeBioprocessing Full time $80,000 - $120,000

    At the forefront of bioprocessing innovation, we are seeking a highly skilled professional to join our team as a Field Applications Scientist. This critical role will require strong technical expertise in cell culture process development, filtration, and manufacturing.The ideal candidate will have excellent communication skills to effectively support...

  • Intern, Upstream Process Development

    5 days ago

    Singapore Hummingbird Bioscience Pte. Ltd Full time

    **Intern, Upstream Process (USP)**: - Hummingbird Bioscience** (Full-time internship - Singapore based location) **ABOUT HUMMINGBIRD BIOSCIENCE** Hummingbird Bioscience is a data-driven precision biotherapeutics team discovering and developing transformative biologic medicines for hard-to-treat diseases. The Hummingbird Bioscience model combines...

  • Senior Scientist, Cell Culture Development

    6 days ago

    Singapore Lonza Biologics Porriño SLU Full time

    Senior Scientist, Cell Culture Development Today, Lonza is a global leader in life sciences operating across three continents. While we work in science, there's no magic formula to how we do it. Our greatest scientific solution is talented people working together, devising ideas that help businesses to help people. In exchange, we let our people own their...