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Upstream Process Development, Scientist I

2 weeks ago

Singapore VALERE SG PTE. LTD. Full time

**Responsibilities**
- Assist in design and execution of cloning experiments, including plasmid construction and transformation.
- Execute studies for pre-CMC and CMC production by evaluating different microbial strains, optimizing fermentation processes, constructing plasmids, banking cells, and conducting microbial fermentations up to 10L scale.
- Gain proficiency in scaling up of processes from shake flasks to bioreactors.
- Support upstream activities which include but are not limited to basic cell culture, cell lysis, media and buffer preparation, and instrument cleaning / maintenance.
- Contribute to the writing of laboratory protocols, standard operating procedures, and other forms of routine documentation.
- Analyze experimental data, including gel electrophoresis and Nanopore sequencing data.
- Effectively communicate experimental data and their implications to internal stakeholders.

**Requirements**:

- Bachelor’s or master's degree in Biological Science and Life Science disciplines, Biotechnology, Bioengineering or related field.
- At least one year of work experience in a bioprocessing, academic or commercial process development setting.
- Familiarity in engineering and culturing _Escherichia Coli _as host for plasmid production.
- Experienced in molecular biology techniques such as DNA extraction and purification, PCR, cloning and assembly, electrophoresis, and transformation.
- Demonstrate proficiency in designing and troubleshooting experiments.
- Work authorization in Singapore.