Aav Development Scientist

**AAV Development Scientist - Manufacturability Group**:
Genetic Design and Magic (GDMC) is a next generation design and manufacturing organization focused on reducing time to clinic and patient for next-gen advanced medicines. We achieve that goal by partnering with and supporting early-stage companies and investigators by bringing our expertise to bear on designing a novel modality and process to be transferable to manufacturing, and then progressing through manufacturing to scale-up for clinical trials in close partnership.

**Responsibilities**
- AAV Strategic responsibilities
- Produce research-grade AAV vectors using the HEK293 system and density gradient-based purification, use of cell stacks, cell factories, small bioreactors
- Enable execution of discovery and pre-clinical activities through the production of research grade AAV vectors.
- Support development of robust, well-characterized, high quality and high yielding downstream production processes for AAV gene therapy products across internal programs.
- Contribute to the design and execution studies to develop a thorough understanding of the process design space, with a view towards scale-up and successful regulatory filings.
- AAV Tactical responsibilities
- Clone highly complex library assemblies for AAV production
- Execute large scale DNA preparation protocols for transfection
- Implement next-gen sequencing DNA preparation procedures
- Set up and manage helper plasmids and AAV vectors inventories
- Co-ordinate production needs with different teams and manage supply chain
- Proactively communicate risks to delivery to management
- Help establish and implement protocols, SOPs and systems within the developing team
- Maintain electronic lab notebooks and communicate results to stakeholders
- Maintain requests dashboard and database
- Maintain production cell lines and their inventory
- Oversee and mentor laboratory technicians and interns in proper laboratory techniques and scientific experimental design
- Manufacturability group standard responsibilities
- Interact with peers across the organization including in the US
- Communicate key finding to internal and external stakeholders
- Assess the manufacturability of a client's modality in conjunction with the rest of the manufacturability team
- Engage collaboratively with the client to understand and improve the manufacturability of therapeutic modalities
- Author and follow standard operating procedures, batch records, and other process documentation
- Filing of invention disclosures and basic understanding of intellectual property with the assistance of relevant internal and external parties/stakeholders
- Drive innovation through the design and troubleshooting of experiments for testing various unit operations in process development in accordance with clients need and company's overall strategic goals

**Requirements**:

- PhD in molecular biology, virology, synthetic biology or equivalent with 1-3 years of laboratory research experience
- 1-5 years of work experience with various AAV systems
- Good understanding of AAV analytics (ddPCR, ELISA, CE-SDS, AUC) in support of product characterization
- Innovative and analytical thinker with outstanding interpersonal, communication, and leadership skill
- Demonstrated ability to clearly communicate technical information and roadmaps through written protocols and/or presentations containing clean figures and visualizations
- Ability to work in BSL2 lab
- Work authorization in Singapore

**Desired Qualifications**
- Strong demonstrable experimental planning/troubleshooting skills
- Hands-on lab knowledge and experience with current and next-gen genome engineering
- and sequencing technologies
- Strong tissue culture skills