Research Fellow

4 days ago

Singapore Yong Loo Lin School of Medicine Full time

**Posting Start Date**:26/07/2024

**Purpose of the post**

The Research Fellow (RF) will be responsible for developing new methodologies utilising high-throughout imaging platforms and single cell RNA sequencing to characterize zoonotic events in simian malaria parasite, _Plasmodium knowlesi_. The RF’s principal role will be to conduct wet lab experiments, data collection and data analysis on the above-mentioned project.
Main

**Responsibilities**:
The main duties and responsibilities are listed below but this is not exhaustive and may evolve as the project and lab requirements develops. The RF will be responsible for:

- Research:

- Establishment of stable _P. knowlesi _malaria cultures in the lab and creating frozen stocks for these. The RF is expected to conduct literature searches regularly and be up to date on the latest research in the field.
- Optimize the protocol for AMNIS Imagestream or similar imaging platforms to capture zoonotic ‘jumps’ in malaria parasites and identify new features to characterize zoonosis.
- Testing the efficiency of different single cell RNA sequencing related protocols (e.g. SMARTseq3, MALBAC, 10X Genomics) that will be most suited for the project aims. Establish an optimized pipeline from start to end for identification of stochastic genes using the optimized approach.
- Sequencing library preparation and outsourcing for sequencing.
- RNA Sequencing data processing and analysis using Unix and R Studio.
Main

**Responsibilities**:
- To organize, document and present results of the experiments in a logical manner.
- Publication preparation and submission.
- To assist in supervising, training and guiding new undergraduate/graduate students/RA in the lab.
- Maintain the highest standard of professional conduct and record keeping in accordance with NUS policies and procedures;
- Laboratory:
Assisting the PI in drafting SOPs, RAs and permit approvals related and required for the above zoonotic malaria project.

**Qualifications**

The applicant should:

- Have a PhD degree in Life Sciences/ Molecular Biology/ Cell Biology/ Biochemistry/ Biotechnology or related area.
- Good command of English language (proficient level speaking, reading, writing) and communication skills.
- Have knowledge of cell and molecular biology techniques such as sterile cell culture, PCR, Reverse transcription, flow cytometry, DNA/RNA purification, fluorescence microscopy. Prior experience in malaria research is highly preferred.
- Knowledge of sequencing sample preparation and data analysis.
- Be able to lead the project and work independently, have an investigative nature, attention to detail;
Application- A detailed CV (including personal particulars, academic and employment history, past research experience).
- Complete list of publications/oral presentations
- Current/expected salary

**More Information**

Location: Kent Ridge Campus

Organization: Yong Loo Lin School of Medicine

Department : Microbiology And Immunology

Employee Referral Eligible: No

Job requisition ID : 25830

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