PCR Microbiologist

2 weeks ago

Singapore ASTAR LABORATORY PTE. LTD. Full time

Duties and Responsibilities 1. Microbiology Responsibilities Conduct microbiological testing on water, food, and environmental samples following approved standards (e.g., ISO, APHA, ASTM). Prepare and maintain culture media, reagents, standards, and required laboratory equipment. Identify, isolate, and quantify microorganisms using conventional and rapid techniques. Support environmental monitoring, including air and surface sampling. Maintain accurate documentation of results, observations, and quality records with proper audit trails. Participate in equipment calibration, method validation, proficiency testing, and quality control programs. Maintain laboratory hygiene and comply with biosafety and ISO/IEC 17025 requirements. Assist in developing and validating new microbiological test methods and workflows. Contribute to continuous improvement of laboratory efficiency, workflow, and technical performance. Provide technical input for troubleshooting, reporting, and addressing the client. 2. PCR Responsibilities Perform qualitative and quantitative PCR (qPCR/ddPCR) assays for DNA/RNA detection in non-clinical samples. Handle low-risk wastewater and environmental samples potentially containing viral genetic material. Conduct manual and automated DNA/RNA extraction and purification. Establish, implement, and maintain PCR protocols and best practices. Identify workflow gaps, equipment needs, and opportunities to improve throughput. Prepare, handle, and store PCR reagents, consumables, and samples appropriately. Monitor PCR consumables and PPE stock levels and coordinate replenishment. Evaluate PCR results and identify potential issues such as inhibition, extraction errors, or amplification anomalies. Perform routine maintenance and basic troubleshooting of PCR instruments and related equipment. Qualifications 1 to 3 years of experience in PCR analysis and method development (qPCR/ddPCR; viral RNA/DNA preferred). Degree in Microbiology, Biomedical Science, Life Science, Biochemistry, or equivalent (relevant experience may substitute). Experience in microbiological laboratory testing is an added advantage. Strong analytical and problem-solving skills with attention to detail. #J-18808-Ljbffr

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