Research Assistant

Found in: Talent SG C2 - 2 weeks ago


Singapur, Singapore Nanyang Technological University Full time

The National Institute of Education invites suitable applications for the position of Research Assistant on a 12-month contract at the .

Project Title: Marine Organisms as Sources of Specialized Metabolites to Disrupt Bacterial Communication: Isolation and Identification of Quorum Sensing Inhibitors

Project Introduction: The emergence and the spread of multidrug-resistant bacteria constitute a major public health issue. Among incriminated Gram-negative bacteria, has been designated by the WHO as a critical priority threat. has the capacity to form biofilms, reinforcing its virulence and intrinsic drug resistance. The regulation of biofilm and virulence factor production of this microorganism is controlled by a specific bacterial communication system called Quorum Sensing (QS). The development of antivirulence agents targeting QS that could attenuate pathogenicity without affecting its growth is therefore an attractive new therapeutic strategy. The current research proposal aims to identify unique specialized metabolites as QS inhibitors (QSIs) from largely underexplored local marine fauna and flora, specifically marine sponges, octocorals and cyanobacteria found at southern islands of Singapore. The proposal has four main objectives: (A) collection and screening of selected marine taxonomic groups for QS inhibitory activities using reporter strains, (B) perform MS-based metabolomic analysis of bioactive fractions for compound dereplication and detection of new/novel metabolites, (C) purification and structural determination of bioactive molecules using chromatographic and spectroscopic methods and (D) evaluation of compounds in attenuating virulence factors production and biofilm formation in with collaborators at SCELSE, NTU.

Requirements:

Possess a BSc degree in Chemistry/Biology-related fields

Be familiar with experimental techniques pertaining to organic chemistry and/or microbiology

Ability to work independently

Strong written and verbal communication skills

Well-rounded and mature character

Good interpersonal and organizational skills

Responsibilities:

Assist in field collection of marine samples

Carry out extractions of marine samples

Carry out semisynthesis of organic molecules and microbiology-related bioassays

Perform data analysis

Other tasks as assigned by the Principal Investigator

Application

Applicants (external and internal) will apply via Workday. We regret that only shortlisted candidates will be notified.

Closing Date

Closing date for advertisements will be set to 14 calendar days from date of posting.

Hiring Institution: NIE
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