Scientist I/II

4 days ago

Singapore A*STAR RESEARCH ENTITIES Full time

We are seeking a highly motivated and technically skilled scientist to join our EDDC Structural Biology team. The successful candidate will play a key role in driving protein production to support biophysical characterization and structural biology efforts within drug discovery programs. This position requires an independent, solution-oriented scientist who can contribute to research projects and cross-functional collaborations. Experience in biophysics and structural biology will be considered a strong advantage. Key Responsibilities Establish and execute recombinant protein expression, purification, and characterization workflows for both soluble and membrane proteins using prokaryotic, eukaryotic, and cell-free systems. Apply your scientific expertise to troubleshoot challenges and propose innovative solutions that drive experimental success. Collaborate across teams to optimize workflows and scale up pipelines for structural biology and biophysical characterization. Play a central role in coordinating protein production activities, ensuring high standards in data integrity, reproducibility, and best practices. Produce high-quality protein samples to support biophysical assays and structure determination efforts. Contribute to a collaborative and knowledge-sharing culture across functional groups within EDDC. Qualifications and Experience Ph.D. or Master's degree in Biochemistry, Structural Biology, Biophysics, Molecular Biology, or a related field, with 3 - 6 years of relevant experience in academic or industry settings. Proven expertise in recombinant protein expression and purification using E. coli, insect, or mammalian systems. Hands-on experience with chromatography systems (e.g., ÄKTA or equivalent). Demonstrated ability to independently plan experiments, analyze data, and deliver results under tight timelines. Experience with biophysical characterization techniques (e.g., MST, BLI, ITC, DSF) and structural biology methods is a strong advantage. Skills and Attributes Strong foundation in protein engineering, including tagging strategies and construct design. Familiarity with high-throughput systems for protein expression and purification, including small-scale screening approaches. A proactive and adaptable mindset, with a collaborative spirit and a passion for solving complex scientific challenges. Ability to thrive in a fast-paced, multidisciplinary environment and contribute meaningfully to team goals. #J-18808-Ljbffr

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